modesticaldum strain ice1  (ATCC)

Journal: Applied and Environmental Microbiology

Article Title: A Molecular Biology Tool Kit for the Phototrophic Firmicute Heliobacterium modesticaldum

doi: 10.1128/AEM.01287-19

Figure Lengend Snippet: Systematic determination of vitamin requirements for H. modesticaldum a

Article Snippet: E. coli cultures were grown in mPYE or Luria-Bertani (LB) broth (BD Difco 244610) supplemented with 100 μg/ml erythromycin and/or 15 μg/ml chloramphenicol, as appropriate, or on LB plates (1.5% agar) supplemented with 300 μg/ml erythromycin and/or 30 μg/ml chloramphenicol, as appropriate. table ft1 table-wrap mode="anchored" t5 TABLE 5 caption a7 Strain or plasmid Description Source Strains Heliobacterium modesticaldum Ice1 (ATCC 51547) Originally isolated from hot springs soil in Iceland ( 8 ) Gift from Michael Madigan E. coli S17-1 Mobilization host containing chromosomally integrated tra genes ATCC 47055 NEBα Cloning strain NEB TOP10 Cloning strain Invitrogen Plasmids pBAD33 Low-copy-number vector containing arabinose-inducible promoter ATCC 87402 pPB347 pBAD33 bearing HM1_2858, HM1_3075, HM1_3076, HM1_3037, and HM1_3004 This study pJIR1457 E. coli-C. perfringens shuttle vector Gift from Joseph A. Sorg ( 21 ) pMTL82251 Modular plasmid with pBP1 replication module Chain Biotech ( 23 ) pMTL83251 Modular plasmid with pCB102 replication module Chain Biotech ( 23 ) pMTL84251 Modular plasmid with pCD6 replication module Chain Biotech ( 23 ) pMTL85251 Modular plasmid with pIM13 replication module Chain Biotech ( 23 ) pMTL86251 Modular plasmid with pIP404 replication module Chain Biotech ( 23 ); this study Open in a separate window Strains and plasmids used in this study Determination of MICs of antibiotics.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: A Molecular Biology Tool Kit for the Phototrophic Firmicute Heliobacterium modesticaldum

doi: 10.1128/AEM.01287-19

Figure Lengend Snippet: MICs for H. modesticaldum grown on mPYE plates a

Article Snippet: E. coli cultures were grown in mPYE or Luria-Bertani (LB) broth (BD Difco 244610) supplemented with 100 μg/ml erythromycin and/or 15 μg/ml chloramphenicol, as appropriate, or on LB plates (1.5% agar) supplemented with 300 μg/ml erythromycin and/or 30 μg/ml chloramphenicol, as appropriate. table ft1 table-wrap mode="anchored" t5 TABLE 5 caption a7 Strain or plasmid Description Source Strains Heliobacterium modesticaldum Ice1 (ATCC 51547) Originally isolated from hot springs soil in Iceland ( 8 ) Gift from Michael Madigan E. coli S17-1 Mobilization host containing chromosomally integrated tra genes ATCC 47055 NEBα Cloning strain NEB TOP10 Cloning strain Invitrogen Plasmids pBAD33 Low-copy-number vector containing arabinose-inducible promoter ATCC 87402 pPB347 pBAD33 bearing HM1_2858, HM1_3075, HM1_3076, HM1_3037, and HM1_3004 This study pJIR1457 E. coli-C. perfringens shuttle vector Gift from Joseph A. Sorg ( 21 ) pMTL82251 Modular plasmid with pBP1 replication module Chain Biotech ( 23 ) pMTL83251 Modular plasmid with pCB102 replication module Chain Biotech ( 23 ) pMTL84251 Modular plasmid with pCD6 replication module Chain Biotech ( 23 ) pMTL85251 Modular plasmid with pIM13 replication module Chain Biotech ( 23 ) pMTL86251 Modular plasmid with pIP404 replication module Chain Biotech ( 23 ); this study Open in a separate window Strains and plasmids used in this study Determination of MICs of antibiotics.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: A Molecular Biology Tool Kit for the Phototrophic Firmicute Heliobacterium modesticaldum

doi: 10.1128/AEM.01287-19

Figure Lengend Snippet: Agarose gel of plasmid minipreps from H. modesticaldum using modified and unmodified isolation procedures. Lanes 1 and 7, 1-kb ladder (NEB, Ipswich, MA); lanes 2 to 5, HindIII-digested plasmid minipreps from H. modesticaldum tcg cells bearing pMTL86251, using the unmodified (lanes 2 and 3) and modified (lanes 4 and 5) spin column procedure; lane 6, plasmid miniprep (unmodified) of pMTL86251 from E. coli TOP10 cells.

Article Snippet: E. coli cultures were grown in mPYE or Luria-Bertani (LB) broth (BD Difco 244610) supplemented with 100 μg/ml erythromycin and/or 15 μg/ml chloramphenicol, as appropriate, or on LB plates (1.5% agar) supplemented with 300 μg/ml erythromycin and/or 30 μg/ml chloramphenicol, as appropriate. table ft1 table-wrap mode="anchored" t5 TABLE 5 caption a7 Strain or plasmid Description Source Strains Heliobacterium modesticaldum Ice1 (ATCC 51547) Originally isolated from hot springs soil in Iceland ( 8 ) Gift from Michael Madigan E. coli S17-1 Mobilization host containing chromosomally integrated tra genes ATCC 47055 NEBα Cloning strain NEB TOP10 Cloning strain Invitrogen Plasmids pBAD33 Low-copy-number vector containing arabinose-inducible promoter ATCC 87402 pPB347 pBAD33 bearing HM1_2858, HM1_3075, HM1_3076, HM1_3037, and HM1_3004 This study pJIR1457 E. coli-C. perfringens shuttle vector Gift from Joseph A. Sorg ( 21 ) pMTL82251 Modular plasmid with pBP1 replication module Chain Biotech ( 23 ) pMTL83251 Modular plasmid with pCB102 replication module Chain Biotech ( 23 ) pMTL84251 Modular plasmid with pCD6 replication module Chain Biotech ( 23 ) pMTL85251 Modular plasmid with pIM13 replication module Chain Biotech ( 23 ) pMTL86251 Modular plasmid with pIP404 replication module Chain Biotech ( 23 ); this study Open in a separate window Strains and plasmids used in this study Determination of MICs of antibiotics.

Techniques: Agarose Gel Electrophoresis, Plasmid Preparation, Modification, Isolation

Journal: Applied and Environmental Microbiology

Article Title: A Molecular Biology Tool Kit for the Phototrophic Firmicute Heliobacterium modesticaldum

doi: 10.1128/AEM.01287-19

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: E. coli cultures were grown in mPYE or Luria-Bertani (LB) broth (BD Difco 244610) supplemented with 100 μg/ml erythromycin and/or 15 μg/ml chloramphenicol, as appropriate, or on LB plates (1.5% agar) supplemented with 300 μg/ml erythromycin and/or 30 μg/ml chloramphenicol, as appropriate. table ft1 table-wrap mode="anchored" t5 TABLE 5 caption a7 Strain or plasmid Description Source Strains Heliobacterium modesticaldum Ice1 (ATCC 51547) Originally isolated from hot springs soil in Iceland ( 8 ) Gift from Michael Madigan E. coli S17-1 Mobilization host containing chromosomally integrated tra genes ATCC 47055 NEBα Cloning strain NEB TOP10 Cloning strain Invitrogen Plasmids pBAD33 Low-copy-number vector containing arabinose-inducible promoter ATCC 87402 pPB347 pBAD33 bearing HM1_2858, HM1_3075, HM1_3076, HM1_3037, and HM1_3004 This study pJIR1457 E. coli-C. perfringens shuttle vector Gift from Joseph A. Sorg ( 21 ) pMTL82251 Modular plasmid with pBP1 replication module Chain Biotech ( 23 ) pMTL83251 Modular plasmid with pCB102 replication module Chain Biotech ( 23 ) pMTL84251 Modular plasmid with pCD6 replication module Chain Biotech ( 23 ) pMTL85251 Modular plasmid with pIM13 replication module Chain Biotech ( 23 ) pMTL86251 Modular plasmid with pIP404 replication module Chain Biotech ( 23 ); this study Open in a separate window Strains and plasmids used in this study Determination of MICs of antibiotics.

Techniques: Plasmid Preparation, Isolation, Cloning